Evaluating two methods for fingerprinting genomes of Actinobacillus actinomycetemcomitans.

  title={Evaluating two methods for fingerprinting genomes of Actinobacillus actinomycetemcomitans.},
  author={J\orgen Slots and Y. B. Liu and J M Dirienzo and Casey Chen},
  journal={Oral microbiology and immunology},
  volume={8 6},
The arbitrary primer polymerase chain reaction (AP-PCR) and Southern blot restriction fragment length polymorphism (RFLP) were used to genotype the periodontal pathogen A. actinomycetemcomitans. Total genomic DNA from 73 strains was extracted by conventional methods. Three random-sequence 10-base oligonucleotide primers were chosen for AP-PCR. The amplified DNA products were separated electrophoretically in a 1% agarose gel containing ethidium bromide and the banding patterns were compared… CONTINUE READING

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