Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish

@article{Fisher2006EvaluatingTB,
  title={Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish},
  author={Shannon J. Fisher and Elizabeth A. Grice and Ryan M. Vinton and Seneca L. Bessling and Akihiro Urasaki and Koichi Kawakami and Andrew S. McCallion},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={1297-1305}
}
Evaluating the biological relevance of the myriad putative regulatory noncoding sequences in vertebrate genomes represents a huge challenge. Functional analyses in vivo have typically relied on costly and labor-intensive transgenic strategies in mice. Transgenesis has also been applied in nonrodent vertebrates, such as zebrafish, but until recently these efforts have been hampered by significant mosaicism and poor rates of germline transmission. We have developed a transgenic strategy in… CONTINUE READING
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