Evaluating performance in three-dimensional fluorescence microscopy

@article{Murray2007EvaluatingPI,
  title={Evaluating performance in three-dimensional fluorescence microscopy},
  author={John M. Murray and Paul L. Appleton and Jason R. Swedlow and Jennifer C Waters},
  journal={Journal of Microscopy},
  year={2007},
  volume={228},
  pages={390 - 405}
}
In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including techniques such as 3-D deconvolution and confocal. There has been a great deal of interest and some confusion about which of these methods is 'better', in principle or in practice. The motivation for the experiments reported here is to establish… CONTINUE READING

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References

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Showing 1-10 of 14 references

Optimization of spinning disk confocal microscopy: synchronization with the ultra-sensitive EMCCD. ThreeDimensional and Multidimensional Microscopy: Image Acquistion and Processing XI (ed

  • F. K. Chong, C. G. Coates, D. J. Denvir, N. McHale, K. Thornbury, M. Hollywood
  • 2004
Highly Influential
10 Excerpts

Fluorophores for confocal microscopy: Photophysics and photochemistry

  • R. Y. Tsien, A. Waggoner
  • Handbook of Biological Confocal Microscopy, 2nd…
  • 1995
Highly Influential
1 Excerpt

Practical Flow Cytometry

  • H. M. Shapiro
  • 2003
2 Excerpts

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