Evaluating performance in three-dimensional fluorescence microscopy

  title={Evaluating performance in three-dimensional fluorescence microscopy},
  author={John M. Murray and Paul L. Appleton and Jason R. Swedlow and Jennifer C Waters},
  journal={Journal of Microscopy},
  pages={390 - 405}
In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including techniques such as 3-D deconvolution and confocal. There has been a great deal of interest and some confusion about which of these methods is 'better', in principle or in practice. The motivation for the experiments reported here is to establish… CONTINUE READING


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