Evaluating a set of reference genes for expression normalization in multiple tissues and skeletal muscle at different development stages in pigs using quantitative real-time polymerase chain reaction.

@article{Zhang2012EvaluatingAS,
  title={Evaluating a set of reference genes for expression normalization in multiple tissues and skeletal muscle at different development stages in pigs using quantitative real-time polymerase chain reaction.},
  author={Jing Zhang and Zhonglin Tang and Ning Wang and Liangqi Long and Kui Li},
  journal={DNA and cell biology},
  year={2012},
  volume={31 1},
  pages={
          106-13
        }
}
Gene expression analysis requires the use of reference genes consistently expressed under various conditions. In many cases, however, the commonly used reference genes are not uniformly expressed independently of tissues or environmental conditions. To provide a set of reliable reference genes in pigs, we used quantitative polymerase chain reaction to examine expression of six common reference genes (GAPDH, ACTB, H3F3A, HPRT1, RPL32, and RPS18) in adult tissues and prenatal skeletal muscles at… CONTINUE READING
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