Evaluating a multigene environmental DNA approach for biodiversity assessment

  title={Evaluating a multigene environmental DNA approach for biodiversity assessment},
  author={Alexei J. Drummond and Richard D. Newcomb and Thomas R. Buckley and Dong Xie and Andrew Dopheide and Benjamin C. M. Potter and Joseph Heled and Howard Ross and Leah K. Tooman and Stefanie Grosser and Duckchul Park and Nicholas J. Demetras and Mark I. Stevens and James C. Russell and Sandra H. Anderson and Anna L. Carter and Nicola J. Nelson},
BackgroundThere is an increasing demand for rapid biodiversity assessment tools that have a broad taxonomic coverage. Here we evaluate a suite of environmental DNA (eDNA) markers coupled with next generation sequencing (NGS) that span the tree of life, comparing them with traditional biodiversity monitoring tools within ten 20×20 meter plots along a 700 meter elevational gradient.ResultsFrom six eDNA datasets (one from each of 16S, 18S, ITS, trnL and two from COI) we identified sequences from… 

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