Evaluating Strategies to Normalise Biological Replicates of Western Blot Data

@inproceedings{Degasperi2014EvaluatingST,
  title={Evaluating Strategies to Normalise Biological Replicates of Western Blot Data},
  author={Andrea Degasperi and Marc R. Birtwistle and Natalia Volinsky and Jens Rauch and Walter Kolch and Boris N. Kholodenko},
  booktitle={PloS one},
  year={2014}
}
Western blot data are widely used in quantitative applications such as statistical testing and mathematical modelling. To ensure accurate quantitation and comparability between experiments, Western blot replicates must be normalised, but it is unclear how the available methods affect statistical properties of the data. Here we evaluate three commonly used normalisation strategies: (i) by fixed normalisation point or control; (ii) by sum of all data points in a replicate; and (iii) by optimal… CONTINUE READING
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