Evaluating Gene Expression in C57BL/6J and DBA/2J Mouse Striatum Using RNA-Seq and Microarrays

@inproceedings{Bottomly2011EvaluatingGE,
  title={Evaluating Gene Expression in C57BL/6J and DBA/2J Mouse Striatum Using RNA-Seq and Microarrays},
  author={Daniel Bottomly and Nicole A. R. Walter and Jessica Ezzell Hunter and Priscila Darakjian and Sunita Kawane and Kari J. Buck and Robert P. Searles and Michael Mooney and Shannon K. McWeeney and Robert Hitzemann},
  booktitle={PloS one},
  year={2011}
}
C57BL/6J (B6) and DBA/2J (D2) are two of the most commonly used inbred mouse strains in neuroscience research. However, the only currently available mouse genome is based entirely on the B6 strain sequence. Subsequently, oligonucleotide microarray probes are based solely on this B6 reference sequence, making their application for gene expression profiling comparisons across mouse strains dubious due to their allelic sequence differences, including single nucleotide polymorphisms (SNPs). The… CONTINUE READING
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C57BL/6J ( B6 ) and DBA/2J ( D2 ) are two of the most commonly used inbred mouse strains in neuroscience research .
C57BL/6J ( B6 ) and DBA/2J ( D2 ) are two of the most commonly used inbred mouse strains in neuroscience research .
C57BL/6J ( B6 ) and DBA/2J ( D2 ) are two of the most commonly used inbred mouse strains in neuroscience research .
Subsequently , oligonucleotide microarray probes are based solely on this B6 reference sequence , making their application for gene expression profiling comparisons across mouse strains dubious due to their allelic sequence differences , including single nucleotide polymorphisms ( SNPs ) .
Subsequently , oligonucleotide microarray probes are based solely on this B6 reference sequence , making their application for gene expression profiling comparisons across mouse strains dubious due to their allelic sequence differences , including single nucleotide polymorphisms ( SNPs ) .
Subsequently , oligonucleotide microarray probes are based solely on this B6 reference sequence , making their application for gene expression profiling comparisons across mouse strains dubious due to their allelic sequence differences , including single nucleotide polymorphisms ( SNPs ) .
The current study compares RNA - Seq ( Illumina GA IIx ) with two microarray platforms ( Illumina MouseRef-8 v2.0 and Affymetrix MOE 430 2.0 ) to detect differential striatal gene expression between the B6 and D2 inbred mouse strains .
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