Europium(III) cryptate: a fluorescent label for the detection of DNA hybrids on solid support.

@article{Prat1991EuropiumIIICA,
  title={Europium(III) cryptate: a fluorescent label for the detection of DNA hybrids on solid support.},
  author={O Prat and Evelyne Lopez and G{\'e}rard Mathis},
  journal={Analytical biochemistry},
  year={1991},
  volume={195 2},
  pages={283-9}
}
We report here a new detection method for DNA hybrids on dot blots. The process utilizes DNA or oligonucleotide probes labeled with biotin, followed by recognition with a conjugate of streptavidin and europium cryptate, a time-resolved fluorescent label. Unlike the other lanthanide chelates, this label is an organic molecule embedding a europium ion into an… CONTINUE READING