European validation of a real-time PCR-based method for detection of Listeria monocytogenes in soft cheese.

@article{Gianfranceschi2014EuropeanVO,
  title={European validation of a real-time PCR-based method for detection of Listeria monocytogenes in soft cheese.},
  author={Monica Virginia Gianfranceschi and David Rodr{\'i}guez-L{\'a}zaro and Marta Hern{\'a}ndez and Patricia Gonz{\'a}lez-Garc{\'i}a and Damiano Comin and Antonietta Gattuso and Elisabetta Delibato and Michele Sonnessa and Fr{\'e}d{\'e}rique Pasquali and Vincenza Annunziata Prencipe and Zuzsanna Sreter-Lancz and Mar{\'i}a-Jos{\'e} S{\'a}iz-Abajo and Javier P{\'e}rez-De-Juan and Javier Butr{\'o}n and Lidija Koza{\vc}inski and Danijela Horvatek Tomi{\'c} and Nevijo Zdolec and Gro S. Johannessen and D{\vz}iuginta Jako{\vc}iūnė and John Elmerdahl Olsen and Paola de Santis and Sarah Lovari and Barbara Bertasi and Enrico Pavoni and Antonella Paiusco and Alessandra De Cesare and Gerardo Manfreda and Dario De Medici},
  journal={International journal of food microbiology},
  year={2014},
  volume={184},
  pages={
          128-33
        }
}
The classical microbiological method for detection of Listeria monocytogenes requires around 7 days for final confirmation, and due to perishable nature of RTE food products, there is a clear need for an alternative methodology for detection of this pathogen. This study presents an international (at European level) ISO 16140-based validation trial of a non-proprietary real-time PCR-based methodology that can generate final results in the following day of the analysis. This methodology is based… Expand
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