Eukaryotic promoters can direct protein synthesis in Gram-negative bacteria.

  title={Eukaryotic promoters can direct protein synthesis in Gram-negative bacteria.},
  author={Sylvie Goussard and Catherine Grillot-Courvalin and Patrice Courvalin},
  journal={Journal of molecular microbiology and biotechnology},
  volume={6 3-4},
Intracellular bacteria can act as DNA delivery vectors into mammalian cells. Transfer of genetic information can be monitored by screening for cellular expression of a reporter gene under the control of an eukaryotic promoter. However, intracellular bacteria can also efficiently deliver heterologous proteins in the cell cytosol. We have studied the activity of the eukaryotic PCMV promoter in Escherichia coli and Salmonella typhimurium using the lacZ and gfp genes as reporters and determined its… CONTINUE READING

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