Eukaryotic expression of recombinant Pso o 1, an allergen from Psoroptes ovis, and its localization in the mite.

@article{Nisbet2007EukaryoticEO,
  title={Eukaryotic expression of recombinant Pso o 1, an allergen from Psoroptes ovis, and its localization in the mite.},
  author={Alasdair J. Nisbet and Anne Mackellar and Kevin McLean and Gerry P. Brennan and John F. Huntley},
  journal={Parasitology},
  year={2007},
  volume={134 Pt 1},
  pages={83-9}
}
A cDNA encoding the immunogen Pso o 1 from Psoroptes ovis was obtained by polymerase chain reaction (PCR) amplification. The amplicon contained the entire coding sequence for the prepro-enzyme in an open reading frame (ORF) of 966 bp. This gene encoded a predicted protein of 322 amino acids (aa) with 64% aa identity (80% similarity) to the major house dust mite faecal allergen Der f 1. The pro-enzyme form of Pso o 1 was expressed as a recombinant protein in the Pichia pastoris-eukaryotic… CONTINUE READING

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