Eukaryotic DNA polymerases.

@article{Hubscher2002EukaryoticDP,
  title={Eukaryotic DNA polymerases.},
  author={Ulrich Hubscher and Giovanni Maga and Silvio Spadari},
  journal={Annual review of biochemistry},
  year={2002},
  volume={71},
  pages={
          133-63
        }
}
Any living cell is faced with the fundamental task of keeping the genome intact in order to develop in an organized manner, to function in a complex environment, to divide at the right time, and to die when it is appropriate. To achieve this goal, an efficient machinery is required to maintain the genetic information encoded in DNA during cell division, DNA repair, DNA recombination, and the bypassing of damage in DNA. DNA polymerases (pols) alpha, beta, gamma, delta, and epsilon are the key… 

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References

SHOWING 1-10 OF 163 REFERENCES
DNA polymerase delta, an essential enzyme for DNA transactions.
TLDR
The current knowledge of Pol delta is summarized and will focus in particular to its structural conservation, its functional tasks in the cell and its interactions with other proteins.
DNA Polymerases: Structural Diversity and Common Mechanisms*
  • T. Steitz
  • Biology
    The Journal of Biological Chemistry
  • 1999
TLDR
Of particular interest are the role of editing in the fidelity of copying, the common enzymatic mechanism of polymerases, and the manners in which different domain structures function in the polymerase reaction in analogous ways.
Error-prone repair DNA polymerases in prokaryotes and eukaryotes.
  • M. Goodman
  • Biology
    Annual review of biochemistry
  • 2002
TLDR
This review is devoted primarily to a discussion of Y-family polymerase members that exhibit error-prone behavior, which poses challenging questions about their roles in targeting and trafficking.
Proliferating cell nuclear antigen: More than a clamp for DNA polymerases
TLDR
The dynamic movement of proliferating cell nuclear antigen on and off the DNA renders this protein an ideal communicator for a variety of proteins that are essential for DNA metabolic events in eukaryotic cells.
DNA replication in eukaryotic cells.
TLDR
This review describes the current understanding of the events of initiation of eukaryotic replication factors and how they are coordinated with cell cycle progression and emphasizes recent progress in determining the function of the different replication factors once they have been assembled at the origin.
DNA polymerase lambda (Pol λ), a novel eukaryotic DNA polymerase with a potential role in meiosis.
TLDR
Northern blotting, in situ hybridizationanalysis and immunostaining showed high levels of Pol l specifically expressed in testis, being developmentally regulated and mainly associ-ated to pachytene spermatocytes, suggesting a potential role ofPol l in DNA repair synthesis associated withmeiosis.
Mutagenesis in eukaryotes dependent on DNA polymerase zeta and Rev1p.
  • C. LawrenceV. Maher
  • Biology
    Philosophical transactions of the Royal Society of London. Series B, Biological sciences
  • 2001
TLDR
It is suggested that Pol zeta is best regarded as a replication enzyme, albeit one that is used only intermittently, that promotes extension at forks the progress of which is blocked for any reason, whether the presence of an unedited terminal mismatch or unrepaired DNA lesion.
Requirement of mammalian DNA polymerase-β in base-excision repair
TLDR
These studies demonstrate that β-polymerase functions specifically in base-excision repair in vivo, and establishes embryonic fibroblast cell lines homozygous for a deletion mutation in the gene encoding DNA polymerase-β.
DNA polymerase ɛ is required for coordinated and efficient chromosomal DNA replication in Xenopus egg extracts
TLDR
It is shown, with the use of a cell-free replication system with Xenopus egg extracts, that Xenopus Polɛ is indeed required for chromosomal DNA replication, and strongly suggest that Polɚ, along with Polα and Polδ, is necessary for coordinated chromosomalDNA replication in eukaryotic cells.
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