Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.

@article{Nonet1987EucaryoticRP,
  title={Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.},
  author={Michael L. Nonet and C Scafe and John P. Sexton and Richard A Young},
  journal={Molecular and cellular biology},
  year={1987},
  volume={7 5},
  pages={
          1602-11
        }
}
We have isolated a yeast conditional mutant which rapidly ceases synthesis of mRNA when subjected to the nonpermissive temperature. This mutant (rpb1-1) was constructed by replacing the wild-type chromosomal copy of the gene encoding the largest subunit of RNA polymerase II with one mutagenized in vitro. The rapid cessation of mRNA synthesis in vivo and the lack of RNA polymerase II activity in crude extracts indicate that the mutant possesses a functionally defective, rather than an assembly… CONTINUE READING

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