EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens

  title={EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens},
  author={Duo Peng and Rick L. Tarleton},
  journal={Microbial Genomics},
Recent development of CRISPR-Cas9 genome editing has enabled highly efficient and versatile manipulation of a variety of organisms and adaptation of the CRISPR-Cas9 system to eukaryotic pathogens has opened new avenues for studying these otherwise hard to manipulate organisms. Here we describe a webtool, Eukaryotic Pathogen gRNA Design Tool (EuPaGDT; available at, which identifies guide RNA (gRNA) in input gene(s) to guide users in arriving at well-informed and… 

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