Ethylenediaminetetraacetic acid in ammonium hydroxide for reducing decalcification time.

@article{Sanderson1995EthylenediaminetetraaceticAI,
  title={Ethylenediaminetetraacetic acid in ammonium hydroxide for reducing decalcification time.},
  author={C. Sanderson and K. Radley and L. Mayton},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  year={1995},
  volume={70 1},
  pages={
          12-8
        }
}
  • C. Sanderson, K. Radley, L. Mayton
  • Published 1995
  • Chemistry, Medicine
  • Biotechnic & histochemistry : official publication of the Biological Stain Commission
  • Ethylenediaminetetraacetic acid (EDTA) solution is used to decalcify bone specimens for histological examination. Sodium hydroxide (NaOH) has been used to dissolve EDTA and to bring EDTA solutions to neutral pH. This solution, however, requires several weeks to decalcify bone specimens. We investigated a new decalcification fluid using concentrated ammonium hydroxide (NH4OH) to dissolve EDTA and to adjust the pH to neutral. Decalcification was performed using a magnetic stirrer with and without… CONTINUE READING
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