• DOI:10.3109/10520299509108310
Corpus ID: 34794514

Ethylenediaminetetraacetic acid in ammonium hydroxide for reducing decalcification time.

@article{Sanderson1995EthylenediaminetetraaceticAI,
  title={Ethylenediaminetetraacetic acid in ammonium hydroxide for reducing decalcification time.},
  author={C. Sanderson and K. Radley and L. Mayton},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  year={1995},
  volume={70 1},
  pages={
          12-8
        }
}

C. Sanderson, K. Radley, L. Mayton

Published 1995

Chemistry, Medicine

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Ethylenediaminetetraacetic acid (EDTA) solution is used to decalcify bone specimens for histological examination. Sodium hydroxide (NaOH) has been used to dissolve EDTA and to bring EDTA solutions to neutral pH. This solution, however, requires several weeks to decalcify bone specimens. We investigated a new decalcification fluid using concentrated ammonium hydroxide (NH4OH) to dissolve EDTA and to adjust the pH to neutral. Decalcification was performed using a magnetic stirrer with and without… CONTINUE READING

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