Ethylene stimulation of latex yield depends on the expression of a sucrose transporter (HbSUT1B) in rubber tree (Hevea brasiliensis).

  title={Ethylene stimulation of latex yield depends on the expression of a sucrose transporter (HbSUT1B) in rubber tree (Hevea brasiliensis).},
  author={Ana{\"i}s Dusotoit-Coucaud and Panida Kongsawadworakul and Laurence Maurousset and Unshira Viboonjun and Nicole Brunel and Val{\'e}rie Pujade-Renaud and Hervé Chrestin and Soulaiman Sakr},
  journal={Tree physiology},
  volume={30 12},
Hevea brasiliensis is an important industrial crop for natural rubber production. Latex biosynthesis occurs in the cytoplasm of highly specialized latex cells and requires sucrose as the unique precursor. Ethylene stimulation of latex production results in high sugar flow from the surrounding cells of inner bark towards the latex cells. The aim of this work was to understand the role of seven sucrose transporters (HbSUTs) and one hexose transporter (HbHXT1) in this process. Two Hevea clones… 

Figures from this paper

Ethylene Response Factors regulate expression of HbSUT3, the sucrose influx carrier in laticifers of Hevea brasiliensis.

Among the five Ethylene Response Factor genes, HbERF-IXc4 showed the strongest transactivation capacity when expressed in tobacco protoplast, the highest transcript abundance in latex, and a close expressional correlation with its target gene, HBSUT3, in response to the Ethrel treatment.

Characterization of a vacuolar sucrose transporter, HbSUT5, from Hevea brasiliensis: involvement in latex production through regulation of intracellular sucrose transport in the bark and laticifers

Insight is provided into the roles of a vacuolar sucrose transporter, HbSUT5, in Suc exchange between lutoids and cytosol in rubber-producing laticifers and thus enhancing the Suc sink strength in laticsifers.

Ethylene Response Factors Are Controlled by Multiple Harvesting Stresses in Hevea brasiliensis

It is suggested that latex harvesting induces mechanisms developed for the response to abiotic stress, probably depend on various hormonal signalling pathways in Hevea.

Structure and expression profile of the sucrose synthase gene family in the rubber tree: indicative of roles in stress response and sucrose utilization in the laticifers

Analysis of the gene structure and phylogeny of the Sus genes demonstrates evolutionary conservation in the Sus families across Hevea and other plant species, especially roles in regulating latex regeneration.

Regulation of HbPIP2;3, a Latex-Abundant Water Transporter, Is Associated with Latex Dilution and Yield in the Rubber Tree (Hevea brasiliensis Muell. Arg.)

expression analysis indicated that the HbPIP2;3 gene was preferentially expressed in latex, and the transcripts were up-regulated by both wounding and exogenously applied Ethrel (a commonly-used ethylene releaser).

Regulation of HbPIP 2 ; 3 , a Latex-Abundant Water Transporter , Is Associated with Latex Dilution and Yield in the Rubber Tree ( Hevea brasiliensis Muell . Arg . )

Rubber tree (Hevea brasiliensis) latex, the source of natural rubber, is synthesised in the cytoplasm of laticifers. Efficient water inflow into laticifers is crucial for latex flow and production

Ethrel® stimulant increases the activity of soluble invertase isoforms in rubber tree (Hevea brasiliensis) bark tissues.

This work demonstrated that this increase in dry rubber production is due to the higher performance of the NIN and VIN invertase isoforms, which contributed to the availability of hexoses in the intracellular compartment favoring rubber biosynthesis and improving the inflow of water necessary to the latex flow.

Transcriptome analysis of Pará rubber tree (H. brasiliensis) seedlings under ethylene stimulation

Investigation of gene expression in response to ethephon treatment using Pará rubber tree seedlings as a model system provides valuable information to understand the mechanism of ethylene stimulation, and will contribute to improved management practices and/or molecular breeding to attain higher yields of latex from Pará Rubber trees.

Molecular mechanism underlying ethylene stimulation of latex production in rubber tree (Hevea brasiliensis)

Key messageEthephon (an ethylene releaser) is commonly used to enhance latex productivity of rubber tree (Hevea brasiliensis), but the underlying mechanism has been elusive. The review presents the



Sucrose importation into laticifers of Hevea brasiliensis, in relation to ethylene stimulation of latex production.

The data and all previous information on sucrose transport show that HbSUT1A and HBSUT2A are related to the increase in sucrose import into laticifers, required for the stimulation of latex yield by ethylene in virgin trees.

Control of carbohydrate metabolism by ethylene in latex vessels ofHevea brasiliensis MUEL. ARC in relation to rubber production

It is concluded that the observed ethylene-induced changes in enzyme aotivities bring about in latex vessels a deviation of the glycolysis-fed metabolio flux from synthetic pathways to mitochondrial oxidations and consequently a decrease in the efficiency of sucrose conversion to rubber.

Sucrose (JrSUT1) and hexose (JrHT1 and JrHT2) transporters in walnut xylem parenchyma cells: their potential role in early events of growth resumption.

It is indicated that xylem parenchyma sucrose transporters make a greater contribution than hexose transporter to the imported carbon supply of bursting vegetative buds.

Insights into Rubber Biosynthesis from Transcriptome Analysis of Hevea Brasiliensis Latex

Both EST and quantitative reverse transcription—PCR(QRTPCR) analyses demonstrated REF and SRPP to be the most abundant transcripts in latexBesides rubber biosynthesis, comparative sequence analysis showed that the RPMPs are highly similar to sequences in the plant kingdom having stress—related functions.

A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seeds.

Feeding high concentrations of sugars suppressed both VfSUT1 expression and transfer cell differentiation in vitro, suggesting a control by carbohydrate availability.

Ethylene-Induced Increase in Glutamine Synthetase Activity and mRNA Levels in Hevea brasiliensis Latex Cells

A specific and significant activation of the cytosolic glutamine synthetase in the laticiferous cells after ethylene treatment parallels the increase of latex yield, and the physiological significance for such a regulation by ethylene of the GScyt is discussed in terms of the nitrogen requirement for protein synthesis associated with latex regeneration.

JrSUT1, a putative xylem sucrose transporter, could mediate sucrose influx into xylem parenchyma cells and be up-regulated by freeze-thaw cycles over the autumn-winter period in walnut tree (Juglans regia L.).

It is proposed that JrSUT1 could be involved in the retrieval of sucrose from xylem vessel with respect to the winter biology of the walnut tree.

Cutting, ageing and expression of plant membrane transporters.

Photosynthate allocation and productivity of latex vessels inHevea brasiliensis

The results stress the importance of photosynthate allocation for the physiology of laticiferous system productivity and indicate the possibilities of improving assimilate economy in rubber trees.

Transcriptome analysis reveals novel features of the molecular events occurring in the laticifers of Hevea brasiliensis (para rubber tree)

The presence of the non-mevalonate DXP/MEP pathway for IPP synthesis in latex was noted by up-regulation of the 1-deoxy-D-xylulose 5-phosphate synthase gene.