Ethylene glycol-supplemented calcium-free media improve zona penetration of vitrified rat oocytes by sperm cells.

@article{Fujiwara2010EthyleneGC,
  title={Ethylene glycol-supplemented calcium-free media improve zona penetration of vitrified rat oocytes by sperm cells.},
  author={Katsuyoshi Fujiwara and Daisuke Sano and Yasunari Seita and Tomo Inomata and Junya Ito and Naomi Kashiwazaki},
  journal={The Journal of reproduction and development},
  year={2010},
  volume={56 1},
  pages={169-75}
}
Cryopreservation of matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies after warming. Even though rats, like mice, have been used in various research fields including reproductive technology, information about cryopreservation of rat oocytes is limited. The objective of the present study was to improve the vitrification protocol for matured rat oocytes. To determine the optimal equilibration time, oocytes were equilibrated in 7.5… CONTINUE READING