Ethanol-tolerant Saccharomyces cerevisiae strains isolated under selective conditions by over-expression of a proofreading-deficient DNA polymerase delta.

@article{Abe2009EthanoltolerantSC,
  title={Ethanol-tolerant Saccharomyces cerevisiae strains isolated under selective conditions by over-expression of a proofreading-deficient DNA polymerase delta.},
  author={Hiroko Abe and Yasuko Fujita and Yuki Takaoka and Eri Kurita and Shuntaro Yano and Naotaka Tanaka and Ken-ichi Nakayama},
  journal={Journal of bioscience and bioengineering},
  year={2009},
  volume={108 3},
  pages={199-204}
}
Ethanol damages the cell membrane and functional proteins, gradually reducing cell viability, and leading to cell death during fermentation which impairs effective bioethanol production by budding yeast Saccharomyces cerevisiae. To obtain more suitable strains for bioethanol production and to gain a better understanding of ethanol tolerance, ethanol-tolerant mutants were isolated using the novel mutagenesis technique based on the disparity theory of evolution. According to this theory evolution… CONTINUE READING

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