Ethanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides.

@article{Nikel2010EthanolSF,
  title={Ethanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides.},
  author={Pablo I. Nikel and Mar{\'i}a Cecilia Ramirez and M Julia Pettinari and Beatriz S. M{\'e}ndez and Miguel Angel Galvagno},
  journal={Journal of applied microbiology},
  year={2010},
  volume={109 2},
  pages={492-504}
}
AIMS Analysis of the physiology and metabolism of Escherichia coli arcA and creC mutants expressing a bifunctional alcohol-acetaldehyde dehydrogenase from Leuconostoc mesenteroides growing on glycerol under oxygen-restricted conditions. The effect of an ldhA mutation and different growth medium modifications was also assessed. METHODS AND RESULTS Expression of adhE in E. coli CT1061 [arcA creC(Con)] resulted in a 1.4-fold enhancement in ethanol synthesis. Significant amounts of lactate were… CONTINUE READING

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