Ethanol Inhibits L1-mediated Neurite Outgrowth in Postnatal Rat Cerebellar Granule Cells*

@article{Bearer1999EthanolIL,
  title={Ethanol Inhibits L1-mediated Neurite Outgrowth in Postnatal Rat Cerebellar Granule Cells*},
  author={Cynthia F. Bearer and Alan R Swick and Mary Ann O'riordan and Gang Cheng},
  journal={The Journal of Biological Chemistry},
  year={1999},
  volume={274},
  pages={13264 - 13270}
}
The neuropathology of the effects of ethanol on the developing central nervous system are similar to those of patients with mutations in L1, a neural cell adhesion molecule. This observation suggests that inhibition of L1 plays a role in the pathogenesis of alcohol-related neurodevelopmental disorders. Here we examine the effects of ethanol on L1 homophilic binding and on L1-mediated neurite outgrowth. Ethanol had no effect on cell adhesion or aggregation in a myeloma cell line expressing full… Expand
Effects of Ethanol and NAP on Cerebellar Expression of the Neural Cell Adhesion Molecule L1
TLDR
Treatment of cerebellar slices with 20 mM ethanol, 10−12 M NAP, or both for 4 hours, 24 hours, and 10 days did not significantly affect L1 mRNA and protein levels; more likely, ethanol disrupts L1 function by modifying its conformation and signaling. Expand
Effects of ethanol on axon outgrowth and branching in developing rat cortical neurons
TLDR
It is found that ethanol does not alter axonal polarization, L1CAM-dependent axon outgrowth or branching, or L 1CAM recycling in axonal growth cones, indicating that ethanol inhibition of L1cAM is highly dependent on neuronal context. Expand
L1 cell adhesion molecule is neuroprotective of alcohol induced cell death.
TLDR
It is concluded that ethanol interferes with processes other than L1-L1 interactions in causing cell death, and that ethanol effects would be more severe in the absence of L1. Expand
Ethanol inhibits neuritogenesis induced by astrocyte muscarinic receptors
TLDR
A potential novel mechanism involved in the developmental effects of ethanol mediated by the interaction of ethanol with cell signaling in astrocytes, leading to an impairment in neuron–astrocyte communication is identified. Expand
Ethanol inhibits L1 cell adhesion molecule tyrosine phosphorylation and dephosphorylation and activation of pp60src
TLDR
It is concluded that ethanol disrupts L1 trafficking/signaling following its expression on the surface of the growth cone, and prior to its activation of pp60src. Expand
Ethanol inhibits muscarinic receptor-induced axonal growth in rat hippocampal neurons.
TLDR
Ethanol inhibited carbachol-induced neurite outgrowth by inhibiting PKC and ERK1/2 activation, which may be responsible for some of the cognitive deficits associated with in utero alcohol exposure. Expand
Ethanol inhibits L1 cell adhesion molecule activation of mitogen‐activated protein kinases
TLDR
It is concluded that ethanol disrupts the signaling pathway between L1 clustering and ERK1/2 activation, and that this occurs independently of the FGFR1 pathway in cerebellar granule cells. Expand
Effect of Lipid Raft Disruption on Ethanol Inhibition of L1 Adhesion
TLDR
Findings indicate that EtOH does not inhibit L1 adhesion in NIH/3T3 cells by inducing the translocation of L1 into lipid rafts. Expand
Ethanol inhibits development of dendrites and synapses in rat hippocampal pyramidal neuron cultures.
TLDR
These results are the first to demonstrate that ethanol has differential effects on axonal and dendritic growth in a culture model of neurons that are vulnerable to ethanol-induced cytoarchitectural abnormalities during development in vivo. Expand
Inhibition of cerebellar granule cell turning by alcohol
TLDR
It is shown that ethanol exposure inhibits the turning of cerebellar granule cells in vivo and in vitro, suggesting that the abnormal allocation of neurons found in the brains of FASD and FSA patients results, at least in part, from impaired turning of immature neurons by alcohol. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 53 REFERENCES
Ethanol enhances neurite outgrowth in primary cultures of rat cerebellar macroneurons.
TLDR
It is indicated that high concentrations of ethanol (150-200 mM) and long periods of exposure (4-7 days) were required to produce toxic effects on neurons and glial cells in this system and that ethanol may have distinct effects on neurite initiation and outgrowth and branching. Expand
Alcohol inhibits cell-cell adhesion mediated by human L1 [published erratum appears in J Cell Biol 1996 Jun;133(5):1139-40]
TLDR
Because L1 plays a role in both neural development and learning, ethanol inhibition of L1-mediated cell-cell interactions could contribute to FAS and ethanol-associated memory disorders. Expand
Ethanol inhibits neural cell-cell adhesion.
TLDR
It is shown that concentrations of ethanol achieved during social drinking inhibit hOP-1-induced cell clustering without affecting cell proliferation, the induction and cell surface expression of N-CAM and L1, or the alternative splicing and sialylation of N -CAM. Expand
Ethanol enhances growth factor-induced neurite formation in PC12 cells
TLDR
The present findings suggest that, in some cells, ethanol directly promotes growth factor-mediated neurite formation, which could harm the nervous system by disturbing the balanced development and organization of synapses. Expand
Ethanol Does Not Inhibit the Adhesive Activity ofDrosophila Neuroglian or Human L1 in DrosophilaS2 Tissue Culture Cells*
TLDR
The previously described inhibition of cell adhesion by ethanol in L1-expressing cells cannot be explained by a simple direct effect on the adhesive activity of L1 family members. Expand
Ethanol inhibits NMDA-stimulated neurite growth by sensory neurons in vitro.
TLDR
Ethanol blocked the NMDA-mediated neurotrophic effect in a manner that was additive with the effects of Mg2+, but non-additive with the inhibitory influences of APV and kynurenate, which have implications for the understanding of adverse influences of ethanol on neuronal development and connectivity. Expand
Calcium influx into neurons can solely account for cell contact- dependent neurite outgrowth stimulated by transfected L1
TLDR
The data demonstrate that whereas NCAM and L1 promote neurite outgrowth via activation of a common CAM-specific second messenger pathway in neurons, neuronal responsiveness to NCAMand L1 is not coordinately regulated via posttranslational processing of NCAM. Expand
Involvement of p90rsk in Neurite Outgrowth Mediated by the Cell Adhesion Molecule L1*
TLDR
The data demonstrate that the membrane-proximal 15 amino acids of the cytoplasmic domain of L1 are important for neurite outgrowth on L1, and the interactions it mediates may be regulated by phosphorylation of Ser1152. Expand
FGF inhibits neurite outgrowth over monolayers of astrocytes and fibroblasts expressing transfected cell adhesion molecules.
TLDR
It is demonstrated that, in certain cellular contexts, FGF can act as an inhibitory cue for axonal growth and that arachidonic acid is the second messenger responsible for this activity. Expand
Deficiency of essential neurotrophic factors in conditioned media produced by ethanol-exposed cortical astrocytes.
  • J. Kim, M. Druse
  • Biology, Medicine
  • Brain research. Developmental brain research
  • 1996
TLDR
It is demonstrated that cultured cortical astrocytes produce trophic factors which are necessary for the development of rhombencephalic neurons, and the damaging effects of in utero ethanol exposure on developing serotonergic neurons may be due to impaired production of astroglial neurotrophic factors. Expand
...
1
2
3
4
5
...