Ethane-freezing/methanol-fixation of cell monolayers: a procedure for improved preservation of structure and antigenicity for light and electron microscopies.

@article{Neuhaus1998EthanefreezingmethanolfixationOC,
  title={Ethane-freezing/methanol-fixation of cell monolayers: a procedure for improved preservation of structure and antigenicity for light and electron microscopies.},
  author={Eva M Neuhaus and Heinz Horstmann and Wolf Almers and Markus Maniak and Thierry Soldati},
  journal={Journal of structural biology},
  year={1998},
  volume={121 3},
  pages={326-42}
}
In order to dissect at the ultrastructural level the morphology of highly dynamic processes such as cell motility, membrane trafficking events, and organelle movements, it is necessary to fix/stop time-dependent events in the millisecond range. Ideally, immunoelectron microscopical labeling experiments require the availability of high-affinity antibodies and accessibility to all compartments of the cell. The biggest challenge is to define an optimum between significant preservation of the… CONTINUE READING

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