Estrogen response in the hFOB 1.19 human fetal osteoblastic cell line stably transfected with the human estrogen receptor gene.

@article{Harris1995EstrogenRI,
  title={Estrogen response in the hFOB 1.19 human fetal osteoblastic cell line stably transfected with the human estrogen receptor gene.},
  author={Samar Harris and K R Tau and Robert J Enger and David O. Toft and B. Lawrence Riggs and Thomas C. Spelsberg},
  journal={Journal of cellular biochemistry},
  year={1995},
  volume={59 2},
  pages={193-201}
}
The gene coding for the human wild-type estrogen receptor (ER) was stably transfected into the human fetal osteoblastic cell line hFOB 1.19, a clonal cell line which is conditionally immortilized with a temperature sensitive mutant of SV40 large T antigen (tsA58). Five subclones were obtained which express various levels of ER mRNA and protein. The subclone with the highest level of functional (nuclear bound) ER, hFOB/ER9, contained 3,931 (+/- 1,341) 17beta-estradiol molecules bound/nucleus as… CONTINUE READING

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