Estradiol metabolism by complementary deoxyribonucleic acid-expressed human cytochrome P450s.

@article{Aoyama1990EstradiolMB,
  title={Estradiol metabolism by complementary deoxyribonucleic acid-expressed human cytochrome P450s.},
  author={Toshifumi Aoyama and Ken Korzekwa and Kiyoshi Nagata and Jennifer Gillette and H. V. Gelboin and Frank J. Gonzalez},
  journal={Endocrinology},
  year={1990},
  volume={126 6},
  pages={3101-6}
}
Twelve forms of human cytochrome P450 were synthesized in human hepatoma Hep G2 cells by means of cDNA-directed expression using vaccinia virus. The cDNA-expressed enzymes were tested for their ability to oxidize estradiol. Incubation of [14C]estradiol with cell lysates containing P450 IA2 resulted in the production of 2-hydroxy and 4-hydroxy metabolites with substrate turnovers of 2.74 and 0.27 min-1, respectively. P450s IIIA3 and IIIA4 yielded the same metabolites at about one third the rate… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 37 extracted citations

The role of CYP2C in the in vitro bioactivation of the contraceptive steroid desogestrel.

The Journal of pharmacology and experimental therapeutics • 1998
View 6 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…