Estradiol-induced mitotic delay in HeLa cells: reversal by calcium chloride and putrescine.

@article{Rao1969EstradiolinducedMD,
  title={Estradiol-induced mitotic delay in HeLa cells: reversal by calcium chloride and putrescine.},
  author={Potu N. Rao},
  journal={Experimental cell research},
  year={1969},
  volume={57 2},
  pages={
          230-4
        }
}
  • P. Rao
  • Published 1 October 1969
  • Biology
  • Experimental cell research

Mitotic inhibition and chromosome displacement induced by estradiol in Chinese hamster cells.

Microtubule experiments showed that DES inhibited spindleAssembly and disassembled the CMTC, whereas estradiol, at similar concentrations, arrested mitosis in a manner that allowed spindle assembly, and changes in cell morphology were observed.

An estradiol mitotic rate inhibiting effect in the Müllerian epithelium in neonatal mice.

  • J. Forsberg
  • Biology, Medicine
    The Journal of experimental zoology
  • 1970
At a continued treatment with estradiol, the mitotic rate values decreased to a level somewhat below the control level in the uterine horns; in the uterus cervix and mullerian vagina theMitotic rate decreased to very low values, significantly lower than in the corresponding regions in the controls.

Proliferative inhibition of human breast carcinoma cells by high concentration estradiol does not alter radiosensitivity

SummaryThe proliferation of ER+ malignant mammary epithelial cells (MMEC), MCF-7 and T-47D, was markedly inhibited by 10µM 17β-estradiol (E2), while the ER− line, MDA-MB-231, was minimally affected.

Accumulation of polyploid cells and G2‐phase cells during ascites tumor growth

These studies indicate that the accumulation of 4C cells during the plateau phase of growth is due to a combination of G2 diploid and G1 tetraploid cells.

THE EFFECT OF ESTRADIOL ON THE CELL KINETICS IN THE UTERINE AND CERVICAL EPITHELIUM OF NEONATAL MICE

  • A. Eide
  • Biology, Medicine
    Cell and tissue kinetics
  • 1975
The effect of estradiol‐17β on the length of the various phases of the cell cycle was studied in the neonatal mouse uterine, and cervical epithelium. A double labelling method was used, and in

Radiation-induced division delay in synchronized Chinese hamster ovary cells in monolayer culture.

Chinese hamster ovary cells in monolayer culture, synchronized by mechanically selecting mitotic cells in the absence of drugs, were x-irradiated with 150, 300, or 600 rads at frequent intervals du...

Cytoplasmic and nuclear growth during the proliferation of Ehrlich ascites tumour cells in mice

There was a continuous enlargement of the average cell size and a consequential change in the cell size distribution and a simultaneous increase in the mean DNA content indicated an accumulation of cells in the postsynthetic period.

Effects of pharmacological concentrations of estrogens on proliferation and cell cycle kinetics of human breast cancer cell lines in vitro.

The cell cycle changes associated with estrogen-induced growth inhibition in vitro are identical to those observed during regression of ER positive but not ER negative human tumor xenografts in nude mice.

References

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Division delay in HeLa cells in Chinese hamster cells. A time-lapse study.

  • G. Froese
  • Biology
    International journal of radiation biology and related studies in physics, chemistry, and medicine
  • 1966
The results suggest that ‘biologically alike’ cells (i.e. sister cells) may show a variation in their division delay; this delay, however, is modified to approach a common value if the cells remain in proximity.

THE MECHANISM OF COLCHICINE INHIBITION OF MITOSIS. I. KINETICS OF INHIBITION AND THE BINDING OF H3-COLCHICINE.

H3-colchicine of high specific activity was prepared and based on the correlation between the time of first appearance of blocked mitoses and the radioactivity per cell, it is suggested that if a critical fraction of the sites are complexed, the cell is unable to form a functional mitotic spindle.

HeLa Cells: Effects of Temperature on the Life Cycle

The period of mitosis is the most temperature-sensitive period of the life cycle of the cell and is a function both of the temperature and of the time which the cell has spent at this temperature.

X-Ray-Induced Incorporation of Tritiated Thymidine into Deoxyribonucleic Acid of Grasshopper Neuroblast Chromosomes

The observation of living neuroblasts exposed to tritiated thymidine and then irradiated in hanging-drop preparations and reidentification of the same cells in autoradiograms of sectioned material show that prophase neuroblast which are delayed, stopped, or reverted mitotically in response to 32 r of X-rays incorporate H/sup 3/T.

THE MECHANISM OF ACTION OF COLCHICINE

The majority of the colchicine-3H bound by tissue culture cells (KB or Hela) was found to be present as a noncovalent complex with a macromolecule which appears in the soluble fraction after