Estimation of the state of the bacterial cell wall by fluorescent In situ hybridization.

@article{Bidnenko1998EstimationOT,
  title={Estimation of the state of the bacterial cell wall by fluorescent In situ hybridization.},
  author={Elena Bidnenko and Carine Mercier and Josselyne Tremblay and Patrick Tailliez and Saulius Kulakauskas},
  journal={Applied and environmental microbiology},
  year={1998},
  volume={64 8},
  pages={
          3059-62
        }
}
Fluorescent in situ hybridization (FISH) is now a widely used method for identification of bacteria at the single-cell level. With gram-positive bacteria, the thick peptidoglycan layer of a cell wall presents a barrier for entry of horseradish peroxidase (HRP)-labeled probes. Therefore, such probes do not give any signal in FISH unless cells are first treated with enzymes which hydrolyze the peptidoglycan. We explored this feature of FISH to detect cells which have undergone permeabilization… CONTINUE READING

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Cell Walls and the Convergent Evolution of the Viral Envelope.

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