Estimation of the lower limit of quantitation, a method detection performance parameter for biomedical assays, from calibration curves.

Abstract

A model for the lower limit of quantitation for biomedical chromatographic assays is proposed. It is based on the IUPAC definition for the limit of detection and can be estimated from assay calibration data. It has been applied to ten different sets of calibration data from various assays of drugs in biological matrices. 

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