Estimation of the homoplasmy degree for transplastomic tobacco using quantitative real-time PCR

@article{Shen2010EstimationOT,
  title={Estimation of the homoplasmy degree for transplastomic tobacco using quantitative real-time PCR},
  author={Huifeng Shen and Bingjun Qian and Litao Yang and Wanqi Liang and Weiwei Chen and Zhenhua Liu and Dabing Zhang},
  journal={European Food Research and Technology},
  year={2010},
  volume={231},
  pages={143-150}
}
Estimation of the homoplasmy of transgene in chloroplast is a necessary step in chloroplast transformation. This task is usually achieved by Southern blot analysis, which is laborious, time-consuming, requires large amounts of plant materials and needs hazardous radioisotopes in some cases. To develop a fast, sensitive, stable and effective technique for determining the homoplasmy of transgene in chloroplast, one real-time PCR system based on TaqMan probe technique was developed for evaluating… CONTINUE READING

Citations

Publications citing this paper.