Estimation of prokaryote genomic DNA G+C content by sequencing universally conserved genes.

@article{Fournier2006EstimationOP,
  title={Estimation of prokaryote genomic DNA G+C content by sequencing universally conserved genes.},
  author={P Fournier and Karsten Suhre and Ghislain Fournous and Didier Raoult},
  journal={International journal of systematic and evolutionary microbiology},
  year={2006},
  volume={56 Pt 5},
  pages={1025-9}
}
Determination of the DNA G+C content of prokaryotic genomes using traditional methods is time-consuming and results may vary from laboratory to laboratory, depending on the technique used. We explored the possibility of extrapolating the genomic DNA G+C content of prokaryotes from gene sequences. For this, 127 universally conserved genes were studied from 50 prokaryotic genomes in the Clusters of Orthologous Groups database. Of these, 57 genes were present as a single copy in the genomes of 157… CONTINUE READING

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