Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems.

@article{Placer1966EstimationOP,
  title={Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems.},
  author={Zd. Placer and L L Cushman and B. Connor Johnson},
  journal={Analytical biochemistry},
  year={1966},
  volume={16 2},
  pages={
          359-64
        }
}
Abstract A new thiobarbituric acid method for the estimation of the malonyl dialdehyde produced as a result of lipid peroxidation is described in which the trimethine colored substance is estimated in alkaline solution. Alkaline pyridine-butanol mixture dissolves both proteins and lipids that are precipitated or opalescent during the reaction, yielding a more accurate MDA estimation as well as simplifying the procedure. 
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References

SHOWING 1-10 OF 14 REFERENCES
Interaction of ascorbic acid and mitochondrial lipides
Abstract Incubation of rat liver mitochondrial suspensions in the presence of low concentrations of ascorbic acid results in the formation of lipide peroxides and the oxidation of the ascorbic acid.Expand
2-Thiobarbituric Acid as a Reagent for Detecting Milk Fat Oxidation
Summary Malonic dialdehyde has been shown to yield a red color when heated with 2-thiobarbituric acid (TBA) reagent. Spectral analysis of this color has revealed it to be identical with that obtainedExpand
ROLE OF ASCORBATE AND CYSTEINE ON SWELLING AND LIPID PEROXIDATION IN RAT LIVER MITOCHONDRIA.
TLDR
Mitochondria partially swollen by l -ascorbate can be contracted with ATP and Mg ++ only if liberated fatty acids are removed by serum albumin and further peroxidation is blocked with EDTA. Expand
The estimation and identification of organic peroxides.
  • J. Philpot
  • Chemistry, Medicine
  • Radiation research
  • 1963
TLDR
It is concluded that irradiation of an animal produces an oxidant, which may be a peroxide or a result of oxidation by aperoxide or an independent product, and that it also produces a deficiency of antioxidant which might be related as either cause or effect, or both, to the production of oxidant. Expand
Addendum: mechansims of lipid peroxide formation in rat tissue homogenates.
  • A. Barber
  • Biology, Medicine
  • Radiation research
  • 1963
TLDR
It was concluded that a complex of inhibitors is available in tissues, and that these inhibitors normally are present in the whole tissue homogenate, as well as in the Whole intact tissue, and it appears that homogenization and dilution break down a certain number of these. Expand
Biochemical implications of pro-oxidants and antioxidants.
TLDR
Analysis of the conditions in intestinal mucosa shows that phospholipase activity can be correlated with antioxidant activity, and the virtual absence of a cofactor reduces the phospholIPase activity and reduces the antioxidant to the same extent after irradiation. Expand
The thiobarbituric acid reaction and the autoxidations of polyunsaturated fatty acid methyl esters.
TLDR
The mechanism of oxidation of methylene-interrupted trienes, tetraenes, pentaenes, and hexaenes postulates an additional step in chain propagation involving intramolecular rearrangement of the hydroperoxy free radical to a free radical bearing a closed five-membered ring peroxide. Expand
The thiobarbituric acid assay of sialic acids.
  • L. Warren
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • 1959
TLDR
This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids. Expand
ON A NEW AEROBIC METABOLITE WHOSE PRODUCTION BY BRAIN IS INHIBITED BY APOMORPHINE, EMETINE, ERGOTAMINE, EPINEPHRINE, AND MENADIONE
1. Suspensions of tissue incubated aerobically produce a substance arbitrarily called B , which can be measured quantitatively by two empirical tests. Evidence is presented suggesting that B containsExpand
Über den Chemismus der Farbreaktion von 2‐Thiobarbitursäure mit Carbonyl‐Verbindungen
Anhand der Ergebnisse systematischer Untersuchungen uber die Reaktion zwischen 2-Thiobarbitursaure (TBS) und definierten, als Modelle ausgewahlten Carbonyl-Verbindungen sowie naturlichen SubstratenExpand
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