Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy.

@article{Tiaho2007EstimationOH,
  title={Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy.},
  author={François Tiaho and Ga{\"e}lle Recher and Denis Rou{\`e}de},
  journal={Optics express},
  year={2007},
  volume={15 19},
  pages={12286-95}
}
We performed Second Harmonic Generation (SHG) imaging microscopy of endogeneous myosin-rich and collagen-rich tissues in amphibian and mammals. We determined the relative components of the macroscopic susceptibility tensor chi((2)) from polarization dependence of SHG intensity. The effective orientation angle theta(e) of the harmonophores has been determined for each protein. For myosin we found theta(e) approximately 62 degrees and this value was unchanged during myofibrillogenesis. It was… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Explore Further: Topics Discussed in This Paper

Citations

Publications citing this paper.
Showing 1-10 of 64 extracted citations

Contrast enhancement in second harmonic imaging : Discriminating between muscle and collagen

Sotiris Psilodimitrakopoulosa, David Artigasa, +5 authors Pablo Loza-Alvarez
2009
View 4 Excerpts
Highly Influenced

Optical second-harmonic images of sacran megamolecule aggregates.

Journal of the Optical Society of America. A, Optics, image science, and vision • 2017

References

Publications referenced by this paper.
Showing 1-10 of 18 references

Implication of the satellite cell in dystrophic muscle fibrosis : a self perpetuating mechanism of collagen over - production

T. Partridge Alexakis, G. Bou-Gharios
Am . J . Physiol . Cell . Physiol . • 2007

Characterization of the myosin - based source for second - harmonic generation from muscle sarcomeres

N. Prent Greenhalgh, C. Green, +3 authors V. Barzda
Biophys . J . • 2006

An SHG magic angle : dependence of second harmonic generation orientation measurements on the width of the orientation distribution

K. L. Rowlen
J . Am . Chem . Soc . • 2004

Studies of χ ( 2 ) / χ ( 3 ) tensors in submicron - scaled bio - tissues by polarization harmonics optical microscopy

W. R. Zipfel, W. W. Webb
Biophys . J . • 2004

Similar Papers

Loading similar papers…