Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy.

  title={Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy.},
  author={François Tiaho and Ga{\"e}lle Recher and Denis Rou{\`e}de},
  journal={Optics express},
  volume={15 19},
We performed Second Harmonic Generation (SHG) imaging microscopy of endogeneous myosin-rich and collagen-rich tissues in amphibian and mammals. We determined the relative components of the macroscopic susceptibility tensor chi((2)) from polarization dependence of SHG intensity. The effective orientation angle theta(e) of the harmonophores has been determined for each protein. For myosin we found theta(e) approximately 62 degrees and this value was unchanged during myofibrillogenesis. It was… CONTINUE READING

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