Estimation of growth fraction with bromodeoxyuridine in human central nervous system tumors.

@article{Yoshii1986EstimationOG,
  title={Estimation of growth fraction with bromodeoxyuridine in human central nervous system tumors.},
  author={Yoshihiko Yoshii and Yutaka Maki and Koji Tsuboi and Yuki Tomono and Keiichi Nakagawa and Takao Hoshino},
  journal={Journal of neurosurgery},
  year={1986},
  volume={65 5},
  pages={659-63}
}
Twenty-five patients with tumors of the central nervous system received bromodeoxyuridine (BUdR), 200 mg/sq m, by intravenous infusion every 8 hours for 3 days before surgery. Excised tumor specimens were fixed in chilled 70% ethanol, embedded in paraffin, and cut into 6-micron sections. Each section was reacted with monoclonal antibodies against BUdR and stained with immunoperoxidase to identify nuclei that had incorporated BUdR. The growth fraction of each tumor was estimated by calculating… CONTINUE READING