Estimation of berberine in ayurvedic formulations containing Berberis aristata.

@article{Rout2008EstimationOB,
  title={Estimation of berberine in ayurvedic formulations containing Berberis aristata.},
  author={Kedar Kumar Rout and Subhalaxmi Pradhan and Sagar Kumar Mishra},
  journal={Journal of AOAC International},
  year={2008},
  volume={91 5},
  pages={1149-53}
}
A sensitive, simple, rapid, and efficient high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for the analysis of berberine in marketed Ayurvedic formulations containing Berberis aristata DC for regulatory purposes. Chromatography of methanolic extracts of these formulations was performed on silica gel 60 F254 aluminum-backed TLC plates of 0.2 mm layer thickness. The plate was developed up to 66 mm with the ternary-mobile phase butanol-acetic acid-water… CONTINUE READING
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