Estimation of Plasma Phosphatase by Determination of Hydrolysed Phenol with Amino-antipyrine

@article{Kind1954EstimationOP,
  title={Estimation of Plasma Phosphatase by Determination of Hydrolysed Phenol with Amino-antipyrine},
  author={P. R. N. Kind and Edward Jasper King},
  journal={Journal of Clinical Pathology},
  year={1954},
  volume={7},
  pages={322 - 326}
}
  • P. Kind, E. King
  • Published 1 November 1954
  • Medicine
  • Journal of Clinical Pathology
Plasma phosphatase was first determined by Martland (1925) and then by Kay (1930), using glycerophosphate and estimating the inorganic phosphate liberated at 370 C. at a specified pH and in a given time. The procedure has been variously modified and simplified by Jenner and Kay (1932), Bodansky (1933), Shinowara, Jones, and Reinhart (1942). Several substrates, other than glycerophosphate, have also been used, e.g., phenyl phosphate (with determination of either the liberated phenol, King and… Expand
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A METHOD FOR THE DETERMINATION OF TARTRATE-LABILE, PROSTATIC ACID PHOSPHATASE IN SERUM
TLDR
The method now described is the result of further work on the tartrate-labile phosphatase component and is a modification of the aminoantipyrine (A.A.P.) methods in use in this laboratory (Kind and King, 1954). Expand
L-Phenylalanine inhibition of human alkaline phosphatases with p-nitrophenyl phosphate as substrate.
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It is suggested that the isoenzyme--inhibitor--substrate complex and the effect of released phosphate on L-phenylalanine inhibition of theIsoenzyme activity differ from each other. Expand
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One new observation appears to be that p -chlorophenylalanine is more inhibitory towards the AP isoenzymes from muscle than towards those from other tissues. Expand
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The results demonstrate that the wide normal range of alkaline phosphatase strictly limits its use as a diagnostic aid, and the one possible exception is that determination of Km appears to have some diagnostic value in the camel. Expand
Perspectives on alkaline phosphatase isoenzymes.
  • W. Fishman
  • Medicine
  • The American journal of medicine
  • 1974
TLDR
In addition to the bone source, isoenzymes of alkaline phosphatase from liver, intestine and placenta have now been found to contribute importantly to the serum, either individually, or in combination, which provides new insights into the mechanism of uncompetitive inhibition. Expand
POTASSIUM AND ACID PHOSPHATASE LEVELS IN SERUM AND PLASMA OF PATIENTS WITH HIGH PLATELET COUNTS
References GERARDE, H. W. (1964), "Unopette Manual or Clinical Laboratory Procedure". Esso Research & Engineering Co., Lindon, New .Tersey, U.S.A. FLEISCHER, .T., WOODSON, G., and SIMET, L. (1956),Expand
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TLDR
Two procedures which substitute a determination of the liberated inorganic phosphate for that of phenol for phosphatase determination are developed, and comparisons of results obtained have shown them to be in excellent agreement. Expand
The Determination of Serum Acid and Alkaline Phosphatase Activity with 4-Aminoantipyrine (A.A.P.)
TLDR
A careful study of the factors which control the development of the colour showed good correlation with those obtained by the method of King (1951) and the use of A.A.P. as a reagent for phenol appeared to be of potential value especially if its use could be extended to the estimation of serum acid phosphatase activity. Expand
A method for the rapid determination of alkaline phosphates with five cubic millimeters of serum.
TLDR
A rapid method has been devised which requires only 5 c.mm. Expand
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