Estimation of Human Norovirus Infectivity from Environmental Water Samples by In Situ Capture RT-qPCR Method

@article{Tian2017EstimationOH,
  title={Estimation of Human Norovirus Infectivity from Environmental Water Samples by In Situ Capture RT-qPCR Method},
  author={Peng Tian and David Yang and Lei Shan and Qianqian Li and Danlei Liu and Dapeng Wang},
  journal={Food and Environmental Virology},
  year={2017},
  volume={10},
  pages={29-38}
}
Human noroviruses (HuNoVs) are highly infectious viruses for which water is an important medium of transmission. In this study, we explored a new in situ capture RT-qPCR (ISC-RT-qPCR) methodology to estimate the infectivity of HuNoV in environmental water samples. This assay was based on capturing encapsidated HuNoV by viral receptors, followed by in situ amplification of the captured viral genomes by RT-qPCR. We demonstrated that the ISC-RT-qPCR did not capture and enable signal amplification… CONTINUE READING
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