Estimating transgene copy number in precocious trifoliate orange by TaqMan real-time PCR

@article{Wen2011EstimatingTC,
  title={Estimating transgene copy number in precocious trifoliate orange by TaqMan real-time PCR},
  author={Li Wen and Bin Tan and Wen-wu Guo},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2011},
  volume={109},
  pages={363-371}
}
Transgene copy number has a great impact on the expression level and stability of exogenous gene in transgenic plants, so transgene copy number analysis is identified as one most important task after obtaining transgenic plants. In this paper, TaqMan real-time PCR was used to estimate the copy number of exogenous MAC12.2 and NPTII genes in transgenic… CONTINUE READING