Estimating the accuracy of polymerase chain reaction-based tests using endpoint dilution.

@article{Hughes2003EstimatingTA,
  title={Estimating the accuracy of polymerase chain reaction-based tests using endpoint dilution.},
  author={James P. Hughes and Patricia A. Totten},
  journal={Biometrics},
  year={2003},
  volume={59 3},
  pages={505-11}
}
Polymerase chain reaction (PCR)-based tests for various microorganisms or target DNA sequences are generally acknowledged to be highly "sensitive," yet the concept of sensitivity is ill-defined in the literature on these tests. We propose that sensitivity should be expressed as a function of the number of target DNA molecules in the sample (or specificity, when the target number is 0). However, estimating this "sensitivity curve" is problematic, since it is difficult to construct samples with a… CONTINUE READING

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