Esterases from Bacillus subtilis and B. stearothermophilus share high sequence homology but differ substantially in their properties

@article{Henke2002EsterasesFB,
  title={Esterases from Bacillus subtilis and B. stearothermophilus share high sequence homology but differ substantially in their properties},
  author={Erik Henke and Uwe Bornscheuer},
  journal={Applied Microbiology and Biotechnology},
  year={2002},
  volume={60},
  pages={320-326}
}
A novel esterase from Bacillus subtilis (BsubE) was cloned, functionally expressed in Escherichia coli and biochemically characterized. BsubE shows high homology (74% identity, >95% homology) to an esterase from the thermophilic B. stearothermophilus (BsteE). Both enzymes were efficiently expressed in E. coli, using a L-rhamnose-expression system [11,500 units/l (BsteE), 3,400 units/l (BsubE)] and were purified by Ni-nitrilotriacetic acid chromatography, yielding specific activities of 70 units… CONTINUE READING