Establishment of two cell lines from embryonic tissue of the tobacco hornworm,Manduca sexta (L.)

@article{Eide1975EstablishmentOT,
  title={Establishment of two cell lines from embryonic tissue of the tobacco hornworm,Manduca sexta (L.)},
  author={P. E. Eide and Jane M. Caldwell and Edwin P. Marks},
  journal={In Vitro},
  year={1975},
  volume={11},
  pages={395-399}
}
Two cell lines were derived from primary cultures of embryonic tissue of the tobacco hornworm,Manduca sexta (L.). The cell lines were maintained on hemolymph-free synthetic insect medium. Cytogenetic and immunological identification of the lines were carried out. Techniques for obtaining the line and medium, subculturing and freezing procedures for long-term storage, and the morphological and growth characteristics are described. 

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Culture organotypique du cerveau de Manduca sexta (Johannson)

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