Establishment of stable cell lines for personalized melanoma cell vaccine.

@article{Selvan2010EstablishmentOS,
  title={Establishment of stable cell lines for personalized melanoma cell vaccine.},
  author={Senthamil R Selvan and Denysha J. Carbonell and Abner W. Fowler and Andrea R Beatty and Mepur H Ravindranath and Robert Owen Dillman},
  journal={Melanoma research},
  year={2010},
  volume={20 4},
  pages={280-92}
}
Personalized vaccine, recognized after the failure of allogenic melanoma whole cell and lysate vaccine phase III trials, involves culturing cells from a patient's own tumor within a short duration and with less passages but with optimized expression of tumor-associated antigens (TAAs). Its feasibility is established by comparing pure cell lines generated from fresh and cryopreserved tissues (n=164) of patients with lymph node (LN) and distant metastases. Stable cell lines (from 67% of specimens… CONTINUE READING
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