Establishment of primary cell culture from the temperate symbiotic cnidarian, Anemonia viridis

@article{BarnayVerdier2013EstablishmentOP,
  title={Establishment of primary cell culture from the temperate symbiotic cnidarian, Anemonia viridis},
  author={S Barnay-Verdier and Diane Dall’Osso and Nathalie Joli and Juliette Olivr{\'e} and Fabrice Priouzeau and Thamilla Zamoum and Pierre-Laurent Merle and Paola Furla},
  journal={Cytotechnology},
  year={2013},
  volume={65},
  pages={697-704}
}
The temperate symbiotic sea anemone Anemonia viridis, a member of the Cnidaria phylum, is a relevant experimental model to investigate the molecular and cellular events involved in the preservation or in the rupture of the symbiosis between the animal cells and their symbiotic microalgae, commonly named zooxanthellae. In order to increase research tools for this model, we developed a primary culture from A. viridis animal cells. By adapting enzymatic dissociation protocols, we isolated animal… CONTINUE READING