Establishment of pluripotent cell lines from porcine preimplantation embryos.

@article{Chen1999EstablishmentOP,
  title={Establishment of pluripotent cell lines from porcine preimplantation embryos.},
  author={L. R. Chen and Y. L. Shiue and Luisa Bertolini and Juan F Medrano and Robert H Bondurant and Gary B. Anderson},
  journal={Theriogenology},
  year={1999},
  volume={52 2},
  pages={195-212}
}
Embryonic stem (ES) cells are pluripotent cells isolated from in vitro culture of preimplantation embryos. Experiments were undertaken to identify preimplantation embryonic stages and culture conditions under which pluripotent, porcine embryo-derived cell lines could be isolated. Cell lines were established from in vitro culture of intact, porcine early hatched blastocysts and isolated inner cell masses (ICM) from intermediate and late hatched blastocysts on feeder layers prepared from… CONTINUE READING
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