[Establishment of indirect ELISA diagnose based on the VP1 structural protein of foot-and-mouth disease virus (FMDV) in pigs].

Abstract

The complete gene encoding the structural protein of FMDV(VP1) was subcloned into expression vector pPROex-HT, resulting in the fusion expression plasmid pPROexHT-VP1. After transformed into E. coli BL21(DE3) and induced by IPTG, the fusion protein was expressed in high level. Western blot was performed to confirm that the expressed fusion protein could specifically react with antiserum against FMDV. Based on the fusion protein further purified, a novel indirect ELISA (VP1-ELISA) was developed to detect FMDV antibody in pigs. Comparison between VPl-ELISA and the government standard kit (liquid phase block ELISA) showed the two methods had 96.25 percent agreement by detecting 80 serum samples, indicating that the indirect VP1-ELISA was specific and sensitive.

Cite this paper

@article{Wang2007EstablishmentOI, title={[Establishment of indirect ELISA diagnose based on the VP1 structural protein of foot-and-mouth disease virus (FMDV) in pigs].}, author={Guanghua Wang and Jun-zheng Du and Guo-zheng Cong and Jun-jun Shao and Tong Lin and Hui-Wen Xue and Hui-yun Chang and Qing-ge Xie}, journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology}, year={2007}, volume={23 5}, pages={961-6} }