Establishment of in vitro spermatogenesis from spermatocytes in the medaka, Oryzias latipes.

@article{Saiki1997EstablishmentOI,
  title={Establishment of in vitro spermatogenesis from spermatocytes in the medaka, Oryzias latipes.},
  author={Akira Saiki and Masato Tamura and Midori Matsumoto and J Katowgi and Akihiko Watanabe and Kazuo Onitake},
  journal={Development, growth & differentiation},
  year={1997},
  volume={39 3},
  pages={337-44}
}
Spermatocytes of the teleost, Oryzias latipes, at meiotic prophase were cultured without contact with somatic cells. They began to divide, progressing through the meiotic divisions and differentiating into round spermatids within 48 h. The chromosome number in both the primary and secondary spermatocytes at metaphase was n = 24. In spermatids, a single flagellum was formed and the release of residual bodies was observed in vitro. The size and shape of the flagellum were the same as those seen… CONTINUE READING
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