Establishment of hairy root lines and analysis of gentiopicroside in the medicinal plant Gentiana macrophylla

  title={Establishment of hairy root lines and analysis of gentiopicroside in the medicinal plant Gentiana macrophylla},
  author={Hong Liang Zhang and Sufang Xue and F. Pu and Rajesh Kumar Tiwari and Xingyun Wang},
  journal={Russian Journal of Plant Physiology},
The leaf explants from the eight-week-old Gentiana macrophylla Pall sterile seedlings were precultured for three days on Murashige and Skoog (MS) agar medium with 1 mg/l of 6-benzyladenine, and then incubated in MS liquid medium containing Agrobacterium rhizogenes R1000 for 2 h with shaking. After 10 days of culture on hormone-free MS agar medium with 500 mg/l cefotaxime under illumination of 300 μmol/(m2 s) with a 16-h photoperiod at 24°C, up to 18.3% of the infected explants produced typical… CONTINUE READING


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