Establishment of cell lines from multipotent epithelial sheet in the budding tunicate, Polyandrocarpa misakiensis.

@article{Kawamura1995EstablishmentOC,
  title={Establishment of cell lines from multipotent epithelial sheet in the budding tunicate, Polyandrocarpa misakiensis.},
  author={Kazuo Kawamura and Shigeki Fujiwara},
  journal={Cell structure and function},
  year={1995},
  volume={20 1},
  pages={97-106}
}
We report here the in vitro culture of the atrial epithelium, which is the major formative tissue of the budding tunicate, Polyandrocarpa misakiensis. Preliminary studies suggested that both pH and osmotic pressure of the basic seawater medium should be lowered significantly (pH 6.8, 800-830 mOsm). In the growth medium consisting of modified millipore-filtered seawater, Dulbecco's modified Eagle medium and 3% fetal bovine serum, cells spread out from the epithelial explant and proliferated with… CONTINUE READING
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