Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA

@inproceedings{Shaked2010EstablishmentOA,
  title={Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA},
  author={Hadassa Shaked and Chaim Wachtel and Pawel Tulinski and Nasreen Hag Yahia and Omer Barda and Edward Darzynkiewicz and Timothy W Nilsen and Shulamit Michaeli},
  booktitle={Nucleic acids research},
  year={2010}
}
In trypanosomes a 39 nucleotide exon, the spliced leader (SL) is donated to all mRNAs from a small RNA, the SL RNA, by trans-splicing. Since the discovery of trans-splicing in trypanosomes two decades ago, numerous attempts failed to reconstitute the reaction in vitro. In this study, a crude whole-cell extract utilizing the endogenous SL RNA and synthetic tubulin pre-mRNA were used to reconstitute the trans-splicing reaction. An RNase protection assay was used to detect the trans-spliced… CONTINUE READING

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