Establishment of an analytical system for the human fecal microbiota, based on reverse transcription-quantitative PCR targeting of multicopy rRNA molecules.

@article{Matsuda2009EstablishmentOA,
  title={Establishment of an analytical system for the human fecal microbiota, based on reverse transcription-quantitative PCR targeting of multicopy rRNA molecules.},
  author={Kazunori Matsuda and Hirokazu Tsuji and Takashi Asahara and Kazumasa Matsumoto and Toshihiko Takada and Koji Nomoto},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 7},
  pages={1961-9}
}
An analytical system based on rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) was established for the precise evaluation of human intestinal microbiota. Group- and species-specific primer sets for Clostridium perfringens, Lactobacillus spp. (six subgroups and three species), Enterococcus spp., and Staphylococcus spp. targeting 16S rRNA gene sequences were newly developed for the quantitative analysis of such subdominant populations in human intestines. They were used together… CONTINUE READING
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