Establishment of an Agrobacterium-mediated transformation system for grape (Vitis vinifera L.): The role of antioxidants during grape–Agrobacterium interactions

@article{Perl1996EstablishmentOA,
  title={Establishment of an Agrobacterium-mediated transformation system for grape (Vitis vinifera L.): The role of antioxidants during grape–Agrobacterium interactions},
  author={Avihai Perl and Ofra Lotan and Mohamad Abu-Abied and Doron Holland},
  journal={Nature Biotechnology},
  year={1996},
  volume={14},
  pages={624-628}
}
Very short exposures of embryogenic calli of Vitis vinifera cv. Superior Seedless grape plants to diluted cultures of Agrobacterium resulted in plant tissue necrosis and subsequent cell death. Antibiotics used for Agrobacterium elimination or as plant selectable markers were not responsible for this necrotic response. Rather, cell death seemed to be oxygen-dependent and correlated with elevated levels of peroxides. Therefore, we studied the effects on necrosis of various combinations of… CONTINUE READING
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