Establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus

  title={Establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus},
  author={Ting-Jun Fan and Bing-Xin Ren and Xiao-Fen Geng and Qiu-Tao Yu and Li-Yan Wang},
A turbot, Scophthalmus maximus, fin (TF) cell line was established and susceptibility to turbot reddish body iridovirus (TRBIV) was determined in this study. Primary culture of TF cells was initiated from fin tissue pieces partially digested with trypsin, collagenase II and hyaluronidase. Digested tissue pieces were cultured at 24 °C in Leibovitz-15 medium (pH 7.2), supplemented with 20% fetal bovine serum, carboxymethyl chitosan, N-acetylglucosamine hydrochloride, basic fibroblast growth… CONTINUE READING
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