Establishment of a suite of assays that support the discovery of proteasome stimulators.


BACKGROUND The proteasome catalyzes the degradation of many mis-folded proteins, which are otherwise cytotoxic. There is interest in the discovery of proteasome agonists, but previous efforts to do so have been disappointing. METHODS The cleavage of small fluorogenic peptides is used routinely as an assay to screen for proteasome modulators. We have… (More)
DOI: 10.1016/j.bbagen.2017.01.003